Table 1.
Production levels of patchoulol and α/β-santalene in the different moss lines, along with ent-16- α-hydroxy-kaurene, stigmasterol, chlorophyll, lutein and carotene.
| Patchoulol | α-santalene | β-santalene | ent-16-α-OH-kaurene | Stigmasterol | Chlorophyll a | Chlorophyll b | Lutein | β-carotene | |
|---|---|---|---|---|---|---|---|---|---|
| WT | 2.42 ± 0.10 | 1.83 ± 0.09 | 27.2 ± 5.63 | 5.57 ± 0.99 | 2.39 ± 0.34 | 2.65 ± 0.43 | |||
| KO | 0 | 1.54 ± 0.03 | 33.3 ± 3.86 | 7.73 ± 0.89 | 2.97 ± 0.35 | 3.36 ± 0.41 | |||
| WT-PTS10 | 0.29 ± 0.04 | 3.23 ± 0.07 | 1.70 ± 0.30 | ||||||
| WT-PTS14 | 0.20 ± 0.10 | 0.16 ± 0.11 | 1.70 ± 0.20 | ||||||
| WT-PTS19 | 0.83 ± 0.08 | 0.61 ± 0.14 | 1.61 ± 0.08 | ||||||
| WT-PTS27 | 0.32 ± 0.04 | 0.39 ± 0.13 | 1.02 ± 0.06 | ||||||
| WT-PTS35 | 0.60 ± 0.04 | 0.22 ± 0.03 | 1.38 ± 0.26 | 24.1 ± 1.43 | 5.98 ± 0.32 | 2.09 ± 0.13 | 2.45 ± 0.26 | ||
| PTS35-S16 | 1.33 ± 0.36 | 0.57 ± 0.17 | 2.13 ± 0.07 | ||||||
| PTS35-S36 | 1.00 ± 0.24 | 0.66 ± 0.13 | 1.87 ± 0.17 | ||||||
| PTS35-S39 | 1.34 ± 0.04 | 0.43 ± 0.09 | 1.79 ± 0.15 | ||||||
| PTS35-KO | 0.38 ± 0.03 | 0 | 2.04 ± 0.07 | 31.5 ± 1.83 | 8.03 ± 0.32 | 2.81 ± 0.09 | 2.76 ± 0.09 | ||
| WT-tpPTS2 | 0.02 ± 0.01 | 1.54 ± 0.14 | 2.00 ± 0.22 | 29.2 ± 5.86 | 6.57 ± 1.22 | 2.58 ± 0.56 | 3.05 ± 0.56 | ||
| WT-STS3 | ND | ND | 3.21 ± 0.55 | 1.52 ± 0.07 | |||||
| WT-STS6 | ND | ND | 1.69 ± 0.37 | 1.23 ± 0.13 | |||||
| WT-STS11 | ND | ND | 0.04 ± 0.02 | 1.55 ± 0.21 | |||||
| WT-STS13 | ND | ND | 1.51 ± 0.50 | 1.21 ± 0.11 | |||||
| STS6-P7 | 0.022 ± 0.011 | 0.020 ± 0.010 | 0.850 ± 0.094 | 1.43 ± 0.38 | |||||
| STS6-P12 | 0.005 ± 0.000 | 0.005 ± 0.000 | 0.075 ± 0.000 | 1.90 ± 0.34 | |||||
| STS6-P14 | 0.010 ± 0.002 | 0.009 ± 0.002 | 0.409 ± 0.039 | 1.35 ± 0.06 | |||||
| STS6-P19 | 0.003 ± 0.001 | 0.003 ± 0.001 | 0.132 ± 0.010 | 1.30 ± 0.16 | |||||
| STS6-P20 | 0.005 ± 0.000 | 0.004 ± 0.000 | 0.520 ± 0.006 | 1.39 ± 0.10 | |||||
| STS6-S17 | 0.005 ± 0.001 | 0.005 ± 0.002 | 0.478 ± 0.044 | 2.13 ± 0.17 | |||||
| STS6-S18 | 0.006 ± 0.001 | 0.005 ± 0.001 | 0.442 ± 0.115 | 2.09 ± 0.48 | |||||
| STS6-S19 | 0.006 ± 0.001 | 0.005 ± 0.001 | 0.344 ± 0.014 | 2.32 ± 0.28 | |||||
| STS6-S29 | 0.005 ± 0.001 | 0.004 ± 0.001 | 0.129 ± 0.010 | 1.61 ± 0.24 | |||||
| WT-tpSTS1 | 0.039 ± 0.008 | 0.035 ± 0.005 | 16.557 ± 5.034 | 1.17 ± 0.05 | 39.1 ± 0.71 | 7.84 ± 0.29 | 3.37 ± 0.25 | 3.88 ± 0.09 |
WT, the wild type line; KO, PpCPS/KS knockout line; WT-PTS/STS, cytosolic patchoulol synthase (PTS)/santalene synthase (STS) lines; PST35/STS6-Sx, SctHMGR-overexpressing PTS/STS lines; STS6-Px, PptHMGR-overexpressing STS lines; PTS35-KO, PpCPS/KS knock out in WT-PST35; WT-tpPTS/tpSTS, plastid targeted PTS/STS lines in WT background; ND, Not Detectable. Unit, mg/g d.w.