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Assessment was based on HNB visualization of color change in (A) and (C) and on gel electrophoresis in (B) and (D). M = 100-bp ladder. In (A) and (B), 1 = 60°C, 2 = 61°C, 3 = 62°C, 4 = 63°C, 5 = 64°C, and 6 = 65°C. In (C) and (D), 1 = 15 min, 2 = 30 min, 3 = 45 min, 4 = 60 min, and 5 = 90 min.
