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. 2014 Nov 18;4:7096. doi: 10.1038/srep07096

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(A). SH-SY5Y-ObRb cells were treated with leptin 0.5 μg/ml for 30 min, and the levels of phospho-STAT3 (Tyr705), STAT3, phospho-ERK1/2 (Thr202/Tyr204), ERK, phospho-Akt (Thr308), Akt, and GAPDH were then analyzed by Western blotting. Leptin increased the phosphorylation of STAT3 (Tyr705), ERK (Thr202/Tyr204), and Akt (Thr308). (B). Densitometric analysis of phospho-STAT3 (Tyr705), phospho-ERK1/2 (Thr202/Tyr204), and phospho-Akt (Thr308) levels using image analyzing software. Data are expressed as the mean ± S.E. of 3 independent experiments (n = 3). ** P < 0.01; *** P < 0.001.