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. 2014 Nov 18;4:7096. doi: 10.1038/srep07096

Figure 4. Leptin induced the phosphorylation of S6K through the PI3K/Akt pathway in SH-SY5Y-ObRb cells.

Figure 4

SH-SY5Y-ObRb cells were pretreated with PD98059 (PD; 10 μM) or LY294002 (LY; 5 μM) for 30 min followed by leptin (0.5 μg/ml, 30 min). Western blotting analysis was performed using specific antibodies for phospho-S6K (Thr389), S6K, and GAPDH. Densitometric analysis of phospho-S6K (Thr389) using image analyzing software. The leptin-induced phosphorylation of S6K was blocked by LY294002. Data are expressed as the mean ± S.E. of 3 independent experiments (n = 3). * P < 0.05.