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. 2014 Nov 18;4:7096. doi: 10.1038/srep07096

Figure 5. The leptin-induced expression of GRP78 was mediated through the mTOR pathway in SH-SY5Y-ObRb cells.

Figure 5

(A). SH-SY5Y-ObRb cells were pretreated with rapamycin (Rapa; 10 nM) prior to the stimulation with leptin (0.5 μg/ml, 30 min). Western blotting analysis was performed using specific antibodies for phospho-S6K (Thr389), S6K, and GAPDH. Densitometric analysis of phospho-S6K (Thr389) using image analyzing software. Rapamycin clearly inhibited leptin-induced S6K phosphorylation. Data are expressed as the mean ± S.E. of 3 independent experiments (n = 3). *** P < 0.001. (B). SH-SY5Y-ObRb cells were pretreated with rapamycin (Rapa; 10 nM) and stimulated with leptin (0.5 μg/ml) for 24 h. Western blotting analysis was performed using antibodies for GRP78 and GAPDH. Densitometric analysis was performed using image analyzing software. Rapamycin significantly blocked the leptin-induced expression of GRP78. Data are expressed as the mean ± S.E. of 3 independent experiments (n = 3). ** P < 0.01.