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. 2014 Nov 18;4:7096. doi: 10.1038/srep07096

Figure 6. Involvement of the PI3K/mTOR pathway on the leptin-induced proliferation of SH-SY5Y-ObRb cells.

Figure 6

SH-SY5Y-ObRb cells were pretreated with LY294002 (LY; 5 μM) or rapamycin (Rapa; 10 nM) for 30 min and then stimulated with leptin (0.5 μg/ml) for 24 h. Cells were incubated with the WST-1 reagent for 4 h and then analyzed for optical density. LY294002 and rapamycin reduced leptin-induced proliferation. Data are expressed as the mean ± S.E. of 4 independent experiments (n = 4). * P < 0.05, ** P < 0.01.