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. 2014 Nov 18;5:627. doi: 10.3389/fpls.2014.00627

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Copyright © 2014 Mustafa and Komatsu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of flooding stress on mRNA expression levels of N-glycosylation related genes. Two-day-old soybeans were treated with flooding for 1 and 2 days (white column). Untreated plants served as a control (black column). RNA extracted from roots of soybean was analyzed by qRT-PCR with specific primers for N-glycosylation genes (Supplemental Table 1; Supplemental Figure 1 for the analyzed samples). mRNA expression levels indicate relative mRNA abundance normalized against 18S rRNA abundance. The data shows mean ± SE values from three independent biological replicates. Among N-glycosylation related genes, glucosaminephosphotransferase (Glyma02g34640.1), alpha-1, 2 glucosyltransferase (Glyma07g15720.1), oligosaccharyltransferase, STT3 subunit (Glyma01g01270.1), and mannosyl-oligosaccharide glucosidase (Glyma05g27890.1) were selected.