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. 2014 Nov 18;5:627. doi: 10.3389/fpls.2014.00627

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Copyright © 2014 Mustafa and Komatsu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of flooding stress on mRNA expression levels of ER related genes. Two-day-old soybeans were treated with flooding for 1 and 2 days (white column). Untreated plants served as controls (black columns). RNA extracted from roots of soybeans was analyzed by qRT-PCR with specific primers for ER protein genes (Supplemental Table 1). mRNA expression levels indicate relative mRNA abundance normalized against 18S rRNA abundance. The data shows mean ± SE values from three independent biological replicates. Among ER related genes, protein disulfide isomerase (Glyma01g25050.1), luminal binding protein 5 (Glyma08g02940.1), and calreticulin (Glyma20g23080.1) were selected.