Table 1.
List of identified glycoproteins of root from 4-day-old and 4-day-old soybean flooded for 2 days compared with 2-day-old soybean.
| Protein ID | Description | M.P. | Ratio 4(0) | Ratio 4(2) | Subcellular localization | Function | N-Glycosylation site | Secretory pathway (Signal P software) | |
|---|---|---|---|---|---|---|---|---|---|
| NetNGlyc software | N-Glycosite software | ||||||||
| 1. Glyma12g09940.2 | FAD/NAD(P) binding oxidoreductase family protein | 5 | 14.20 | 8.17 | Cytoplasm | Not assigned | 27 NVTL, 76 NITE | NVTL, NITET | N |
| 27 NVTL, 76 NITE, 201 | NVTL, NITE, NKTY | N | |||||||
| 2. Glyma11g18320.1 | FAD/NAD(P) binding oxidoreductase family protein | 9 | 9.22 | 8.14 | Cytoplase | Not assigned | NKTY | ||
| 3. Glyma03g31670.1 | RNA binding KH domain containing protein | 2 | 0.67 | 0.89 | Cytoplasm | RNA | 23 NHSD, 424 NISY | NHSD, NISY | N |
| 4. Glyma04g07841.1 | SHK1 binding protein 1 | 2 | 0.61 | 0.70 | Chloroplast | C1-metabolism | 251 NHSI, 547 NDTG | NHSI, NDTG | N |
| 68 NTSL, 146 NHTY, 172 | NTS, NHTY, NGTG, ND S, | N | |||||||
| 5. Glyma13g42320.1 | Lipoxygenase 1 | 7 | 0.49 | 0.69 | Peroxisome | Hormone metabolism | NGTG, 489 NDSC, 641 NDSE | NDSE | |
| 6. Glyma01g42840.1 | Glutathione peroxidase 6 | 4 | 0.40 | 0.64 | Cytoplasm | Redox | 48 NYTE, 132 NFSK | NYT, NFS | N |
| 7. Glyma11g02630.1 | Glutathione peroxidase 6 | 6 | 0.40 | 0.64 | Cytoplasm | Redox | 48 NYTE, 132 NFSK | NYT, NFSK | N |
| 246 NVSD, 340 NFTY, | NVSDA, N FT, N AT, | N | |||||||
| 8. Glyma07g32480.1 | Apoptosis inhibitory protein 5 (API5) | 5 | 0.53 | 0.58 | Cytoplasm | Development | 360 NATN, 416 NKSM, 437 NATT, 469 NLSW, 495 NGSN | NKSMA NATT, NL SW, NGSN | |
| 247 NVSD, 341 NFTY, | NVSD, NFTY, NATN | N | |||||||
| 9. Glyma13g24090.2 | Apoptosis inhibitory protein 5 (API5) | 5 | 0.53 | 0.58 | Cytoplasm | Development | 361 NATN, 417 NKSM, 438 NATT, 470 NLSW, 496 NGSN | NKSM, NAT, N LS, NGSN | |
| 10. | 70 NETS, 106 NSSA, 122 | NETS, NSSA, NHSE, | N | ||||||
| Glyma07g13900.1 | Hyaluronan | 6 | 0.49 | 0.52 | Nucleus | RNA | NHSE 313 NITE | NITE | |
| 11. Glyma07g38790.1 | NAD(P) binding Rossmann fold superfamily protein | 5 | 0.41 | 0.51 | Cytoplasm | Misc | 182 NSTS | NSTS | N |
| 12. Glyma09g25830.2 | CAP160 protein | 11 | 0.17 | 0.50 | Nucleus | Not assigned | 491 NQTT, 917 NQSS | NQTT, NQSS | N |
| 13. Glyma05g04290.1 | Glycosyl hydrolases family 32 protein | 8 | 2.80 | 0.46 | Chloroplast | Major CHO metabolism | 208 NESV, 221 NPSD, 273 NKTG, 616 NATE | NESV, NKTG, NATE | N |
| 14. Glyma13g39790.1 | ABC transporter family protein | 2 | 0.48 | 0.45 | Cytoplasm | Protein.synthesis | 228 NPTI, 496 NLSD | NLSD | N |
| 15. Glyma11g12480.1 | Cold circadian rhythm and RNA binding 2 | 3 | 0.27 | 0.42 | Nucleus | RNA | 78 NITV 104 NRSG | NITV, NRSG | N |
| 16. Glyma11g12490.1 | Cold circadian rhythm and RNA binding 2 | 3 | 0.27 | 0.42 | Cytoplasm | RNA | 78 NITV 135 NGSR | NITV, NGSR | N |
| 24 NASE, 135 NGTT, 278 | NASE, NGTT, NLSL, | N | |||||||
| 17. Glyma03g27030.1 | DNAJ homolog | 2 | 0.46 | 0.39 | Cytoplasm | Stress abiotic | NLSL | ||
| 128 NKTG, 344 NKTA, | NKTG, NKTA, | N | |||||||
| 18. Glyma10g07410.1 | Embryonic cell protein 63 | 6 | 0.35 | 0.23 | Nucleus | Development | 367 NVSG | ||
| 79 NSTT, 92 NLTV, 151 | NSTT, NLTV, NLTE, NFTT, | Y | |||||||
| 19. Glyma12g32160.1 | Peroxidase superfamily protein | 2 | 3.17 | 0.22 | Cytoplasm | Stress abiotic | NLTE 165 NFTT, 206 NFTG, 237 NTTK | NFTG, NTTK | |
| 79 NSTT, 92 NLTV, 165 | NSTT, NLTV, NITT, NFTG, | Y | |||||||
| 20. Glyma12g32170.1 | Peroxidase superfamily protein | 2 | 3.17 | 0.22 | Cytoplasm | Misc | NITT 206 NFTG, 237 NTTK | NTTK | |
| 79 NSTT, 92 NLTV, 165 | NSTT, NLTV, NITT, NFTG, | Y | |||||||
| 21. Glyma13g38300.1 | Peroxidase superfamily protein | 2 | 3.17 | 0.22 | Cytoplasm | Misc | NITT 206 NFTG, 237 NTTK, 292 NFSA | NTTK, NFSA | |
| 77 NSTT, 90 NLTV, 149 | NSTT, NLTV, NLTE, NFTT, | Y | |||||||
| 22. Glyma13g38310.2 | Peroxidase superfamily protein | 2 | 3.17 | 0.22 | Cytoplasm | Stress abiotic | NLTE 163 NFTT, 204 NFTG, 235 NTTK | NFTG, NTTK | |
| 176 NIST, 248 NQSA, 253 | NIST, NQSA, NGTL, | N | |||||||
| 23. Glyma11g10790.1 | Nucleolin like 2 | 8 | 0.31 | 0.03 | Nucleus | Protein synthesis | NGTL 562 NSSN, 567 NNSS, 568 NSSQ | NSSN, NNSS | |
Protein ID, according to the Phytozome database; M.P., matched peptide; Ratio, relative abundance of a protein from 4 day old soybean compared to 2-days old soybean root; ND, no description; N-Glycosylation site, Presence of the N-glycosylation site in protein moiety based on the N-Glycosite Software and NetNGlyc Software. Secretory Pathway, signal peptide presence based on the SignalP 4.1.