Skip to main content
. 2014 May 8;135(6):1381–1389. doi: 10.1002/ijc.28670

Figure 6.

Figure 6

PCR validation of assembled contigs. The assembly process was verified by PCR followed by sequencing. The PCRs were performed on several biopsies: epileptic (Ep), Glioblastoma multiforme (GBM), breast carcinoma brain metastasis (BCBM) and astrocytoma grade II (AII). Amplified DNA products from all samples were visualized on agarose gels with ethidium bromide. PCR amplicons were then extracted and sequenced (DNA Genetic Analyzer 3130XL Applied Biosystems). (a) TIMP1 and (b) α-satellite DNA (α-satDNA) sequence identities were confirmed by NCBI BLAST.