Figure 5. Confidence recurrent subnetwork involved in the KEGG pathways in cancer.

(a) Pan-cancer miRNA-mRNA interactions with posteriors at least 0.2 were used to construct a confidence recurrent network and visualized via Cytoscape²⁴. The subnetwork shown above involved target genes in the KEGG pathways in cancer (KEGG ID: hsa05200)³⁰. The miRNAs and genes are displayed in red diamonds and blue ellipses, respectively, and the edge labels are the posterior probabilities. The yellow highlighted nodes are chosen as two detailed examples described below. (b) We generated the scatterplots to visualize the expression correlation between miR-22-5p and EP300 (left panel) and between miR-107 and TGFB1 (right panel). The corresponding base-paring between the mature miRNAs and the 3′UTR sequences displayed in the bottom were predicted by Smith-Waterman local alignment from EMBOSS suite³².