|
Escherichia coli DH5α |
F− ϕ80 lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rk−, mk+) phoA
supE44 thi-1 gyrA96 relA1 λ−
|
Invitrogen™ |
|
E. coli ET12567 |
dam-13::Tn9 dcm-6 hsdM CmlR, carrying helper plasmid pUZ8002 |
22 |
|
S. coelicolor M1141 |
M145 derivative Δact
|
8 |
|
S. coelicolor M1141Δred_int |
M1141 containing pIJ12740 |
This study |
|
S. coelicolor M1141Δred
|
M1141 ΔredDX
|
This study |
| pUC57-Simple_SceI |
pUC57-Simple with tcp830p-I-SceI gene-fd terminator-MCS-I-SceI site |
GeneScript (Piscataway, USA) |
| pKC1132 |
Conjugative vector, non-replicative in Streptomyces, AprR
|
5 |
| pIJ12738 |
pKC1132 with MCS and I-SceI site from pUC57-Simple_SceI |
This study |
| pGM1190 |
tsr (ThiR), aac(3)IV (AprR), oriT, to terminator, tipAp, RBS, fd terminator |
11 |
| pIJ12739 |
pGM1190 with tipAp-I-SceI gene, ThiR
|
This study |
| pIJ12551 |
Conjugative and ϕC31-integrative vector, AprR
|
19 |
| pIJ12590 |
pIJ12551 with I-SceI gene |
This study |
| pIJ12742 |
pGM1190 with ermE*p-I-SceI gene, ThiR
|
This study |
| pIJ12740 |
pIJ12738 with redXW left and right flanking sequences |
This study |
