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. 2014 Nov 17;55(11):7285–7295. doi: 10.1167/iovs.14-15195

Figure 4.

Figure 4

Cholesteryl esterase Filipin histochemistry of apoB100 and WT mice fed a normal chow diet. (A) 2-month-old WT mouse without blue labeling for cholesteryl esters in BrM (red arrowheads). There is nonspecific staining of the sclera. (B) A 2-month-old apoB100 mouse showing blue stripe labeling for cholesteryl esters in BrM. (C) An 8-month-old WT mouse without labeling for cholesteryl esters in BrM. (D) An 8-month-old apoB100 mouse with blue label for cholesteryl esters in BrM. (E) Section from an 8-month-old apoB100 mouse without Filipin staining shows minimal staining in BrM. (F) Brightfield image of the neurosensory retina (R), RPE, BrM, Ch, and S. Scale bar: 20 μm.