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. Author manuscript; available in PMC: 2014 Nov 18.
Published in final edited form as: Neurobiol Dis. 2014 Jan 14;65:188–192. doi: 10.1016/j.nbd.2013.12.017

Fig. 3.

Fig. 3

Dose-dependent retromer protein deficiency in leucine-supplemented differentiated Neuro2A cells (A–C) Representative Western blots of VPS35, VPS26, VPS34, and tubulin are shown for cells treated with 100% (A), 250% (B), and 500% (C) additional leucine (control = 0.8 mM leucine). Average expression of retromer proteins VPS35 and VPS26 and the PI3KIII Kinase VPS34 in cells supplemented with leucine (black bars) compared to non-supplemented controls (white bars) is summarized (mean ± SEM; n = 6). *P < 0.05, **P < 0.01, ***P < 0.001.