Figure 5.

qPCR for SETDB1 in in vitro- and in vivo-derived embryos. A: SETDB1 abundance decreases from MII oocytes to the blastocyst stage in all treatments. Similar SETDB1 expression was observed in all stages for all treatments. MII oocytes were used as a control for all stages in IVF and SCNT embryos. B: Donor cells have a low SETDB1 abundance, and SCNT and parthenogenetic embryos decrease in abundance during development, returning to a level similar to that of the donor cells. Relative mRNA abundance was normalized to YWHAG. Letters (a, b, etc.) denote differences within a method of production (P < 0.002). Error bars represent standard error.