Table 1.
Homologous AHL-lactonases and AHL-acylases in Photorhabdus spec.
| Strain | Protein | Enzymatic activity | P. luminescens TT01 | P. asymbiotica ATCC43949 | P. temperata NC19 |
|---|---|---|---|---|---|
| B. cereus | AiiA | AHL-lactonase | Plu1749 | Pau1378 | Pte3377 |
| A. tumefaciens | AiiB | AHL-lactonase | – | AttM/AiiB (Pau0481) | – |
| A. tumefaciens | AttM | AHL-lactonase | Plu2238 | AttM/AiiB (Pau0481) | Pte1161 |
| Arthrobacter spec. | AhlD | AHL-lactonase | Plu2238 | – | – |
| P. aeruginosa | PvdQ | AHL-acylase | Plu3527 | – | – |
| Streptomyces spec. | AhlM | AHL-acylase | Plu3527 | – | – |
| D. thermophilum | AhlA | AHL-acylase | Php (Plu1997) | Php (Pau2581) | Pte3570 |
| Anabaena spec. | AiiC | AHL-acylase | Plu3527 | – | – |
The AHL-lactonases AiiA from Bacillus cereus (Wang et al., 2004), AiiB from Agrobacterium tumefaciens (Liu et al., 2007), AttM from Agrobacterium tumefaciens (Zhang et al., 2002) and AhlD from Arthrobacter spec. (Park et al., 2003) were used in this study to identify homologs in the three Photorhabdus species. AHL-lactonases have a “Lactamase_B” domain (PF00144) belonging to the metallo-beta-lactamase superfamiliy. Furthermore, Zn2+-dependent hydrolases exist, e.g., AiiB, AhlD, AttM, QsdA, Plu2238, Pau1378, Pte1161, and Pte3377. The acylases PvdQ from Pseudomonas aeruginosa (Huang et al., 2003), AhlM from Streptomyces spec. (Park et al., 2005), AhlA from Dictyoglomus thermophilum (Coil et al., 2014), and AiiC from Anabaena spec (Romero et al., 2008). Analyses are based on STRING 9.1 database and NCBI Blast (Altschul et al., 1990) were used in this study to identify homologs in the three Photorhabdus species. The AHL-acylases AhlM, AhlA, and AiiC belong to the Ntn-hydrolase superfamily.