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. Author manuscript; available in PMC: 2014 Nov 18.
Published in final edited form as: Circ Heart Fail. 2009 Sep 24;2(6):599–607. doi: 10.1161/CIRCHEARTFAILURE.109.862664

Figure 5. Assessment of apoptosis by TUNEL staining.

Figure 5

A1: TUNEL-positive cells (green fluorescence). A2: DAPI-positive nuclei (blue fluorescence). A3: desmin-positive cells (red fluorescence). A4: color-combined image (all from heart 6 weeks after PAB). Magnification ×200. Arrows indicate TUNEL-positive nuclei. The white scale bar represents 100 microns.

B: Graphs summarize the number of TUNEL-positive nuclei per 1000 cardiomyocyte nuclei, and represent the average from 10 different fields selected randomly. In the septum, more TUNEL-positive nuclei were found in the PAB group than in the control group, especially at 6 weeks after PAB. * P<0.05, and †P<0.01 versus the control group.