FIG. 6.

Characterization of immune response at time of AAV administration in SP-A KO/PAO1 acute infection model. (A–H) Lung histology of SP-A KO mice transduced on day 2 during PAO1 airway infection. Lungs were harvested on the day of AAV administration and sectioned and stained with antibodies against antigens for the specified cell type. Representative images are shown here: sections from (A–D) uninfected mice and (E–H) infected mice. On day 2, elevated levels of neutrophils, macrophages, and CD8 T cells were evident in samples from infected lungs (see arrows). Magnification,×20. (I–J) Cytospin preparations of cells recovered from fresh BALF samples taken on day of AAV administration: (I) samples taken from uninfected mice and (J) samples taken from infected mice. Representative macrophages (Ma) and neutrophils (Ne) are indicated in the figure. Samples from uninfected mice showed only macrophages, while infected samples showed obvious neutrophil infiltration in addition to the macrophage cell population. Color images available online at www.liebertpub.com/hum