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. 2014 Jul 15;23(23):2883–2894. doi: 10.1089/scd.2014.0066

FIG. 2.

FIG. 2.

Presence of a circular odontoblast layer within dental pulp in 1- and 6-day DSPP-KO mice (A–C) and abnormal cusp formation in 1- and 6-day DSPP-KO mice (G–I). Representative samples were derived from six 1-day and six 6-day DSPP-KO mice. The sections were stained with H&E. Panels (A–C) show circular odontoblast layer. (A) Six-day DSPP-KO M1 (100× magnification). (B) One-day DSPP-KO M2 (200× magnification). (C) Six-day DSPP-KO M2 (200× magnification). Note that dentin formed a circular structure, indicated by the box, within the dental pulp in each of these samples and the enamel and dentin layers were abnormally separated. M2, second molar. Scale bar=100 μm. (D–F) Represent normal cusp formation. (D) One-day wt M1. Note intact cusp. (E) Six-day wt M1. Note intact cusp and normal dentin/enamel formation. (F) Six-day wt M2. Note intact cusp and normal dentin/enamel formation. (G–I) Represent abnormal cusp formation. (G) One-day DSPP-KO M1. Note cusp with thin dentin without enamel. (H) Six-day DSPP-KO M1. Note abnormal cusp. The dentin layer in the cusp is thinner than that of wt M1. (I) Six-day DSPP-KO M2. Note abnormal cusp. Scale bar=100 μm for all frames.