FIG. 1.
Proliferation rate of hASCs is growth medium dependent. (A) The graph describes the proliferation capacity of hASCs in Dulbecco's modified Eagle's medium (DMEM) (open symbols) and in EGM-2 medium (closed symbols) [n=3 for each condition; error bars, standard deviation (SD)]. The red horizontal line marks the 1010 cell level. Roman numerals (arrows) indicate the times at which β-galactosidase expression was assayed. (B) β-Galactosidase staining (blue), a senescence marker, shows entry of cells in the stationary growth phase. hASCs, human adipose-tissue-derived MSCs. Color images available online at www.liebertpub.com/scd