FIG. 3.

Safety of FP-ASCs. Luciferase-expressing, early (PD 9) and late (PD 18), FP-ASCs were i.m. implanted in SCID mice and monitored regularly by BLI over a 160-day period. Normal hASCs (PD 10) were also implanted for comparison. (A) The graph represents the luciferase activity recorded from the BLI images of the implantation sites at the indicated days. Data points represent average values±SD (bars) (n=6 for each of the patient-derived cells). (B) Representative image showing a CGH profile from a late-passage FP-ASC patient sample. Shaded areas corresponding to centromeres and constitutive heterochromatin are not considered. The vertical lines, left and right of the CGH profile, represent fluorescence ratio limit values of 0.5–0.75 (red) and 1.0–1.25 (green). CGH ratio values between 1.25 and 0.75 indicate no significant gain or loss of cell DNA relative to control DNA and are considered normal. The actual ratio (black line) between test DNA and control DNA shows no gain or loss of DNA by the chromosomes. Numbers under each diagram indicate chromosome number and the number of chromosomes tested (parenthesis), respectively. SCID, severe-combined immunodeficient mouse; BLI, bioluminescent imaging; CGH, comparative genome hybridization. Color images available online at www.liebertpub.com/scd