Figure 1.
Characterization of vascular remodeling in EAE. A. Clinical progression of EAE, scored as described in Materials and Methods. This experiment included 9 EAE and 3 CFA control mice. Data are expressed as the mean ± SD. B. Quantification of change in total vascular area in cervical spinal cord during development of EAE, as measured by CD31 IF and Volocity software. Experiments were performed with 3 mice per time-point, and the results expressed as the mean ± SEM. * p < 0.05, ** p < 0.01. Note that total vascular area was significantly increased 7 days post-immunization, continued to increase up to 21 days, and thereafter was maintained at greater than 50% higher than control levels. C. Representative CD31 IF images demonstrating increasing vascular density during EAE development. Scale bar =100 μm.
