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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Exp Neurol. 2013 Sep 20;250:43–51. doi: 10.1016/j.expneurol.2013.09.009

Figure 2.

Figure 2

Endothelial cell proliferation during EAE. A. Dual IF for CD31 (Alexa Fluor-488, green) and Ki67 (Cy3, red) was performed on frozen sections of EAE cervical spinal cord taken from 0, 4, 7, 14, and 21 days post-immunization. Scale bar =100 μm. Note that control tissue contains no Ki67+ cells, but these gradually increase with development of EAE, reaching a peak after 14 days. B. CD31/Ki67 dual IF high power images from the 7 and 14 day time-points, showing endothelial cell proliferation within remodeling blood vessels (see arrows). Scale bar = 25 μm. C. Quantification of endothelial cell proliferation during EAE development. Data points represent the mean ± SEM of 3 experiments. Note that endothelial cell proliferation peaks at 14 days post-immunization, then declines. * P<0.05, *** p < 0.01.