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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Exp Neurol. 2013 Sep 20;250:43–51. doi: 10.1016/j.expneurol.2013.09.009

Figure 3.

Figure 3

EAE-induced angiogenesis occurs in myelinated regions of the cervical spinal cord. Adjacent sections of 7 day post-immunized cervical spinal cord were stained with either: (A) MBP (Alexa Fluor-488, green) or (B) CD31 (Alexa Fluor-488, green)/Ki67 (Cy3, red) dual-IF. Scale bar = 100 μm. C shows a magnified inset (scale bar = 50 μm) taken from panel B. D. Further examples of Ki67-positive proliferating endothelial cells within angiogenic blood vessels. Scale bar = 25 μm Note that MBP staining demarcates the white matter (WM), and that within the cervical spinal cord, endothelial cell proliferation occurs predominantly in the myelinated region. E. Quantification of endothelial cell proliferation within the grey and white matter at different stages of EAE development. Data points represent the mean ± SEM of 3 experiments. Note that endothelial cell proliferation occurs predominantly in the white matter, and increases up to the 14 day time-point. * P<0.05, ** p < 0.01.