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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Exp Neurol. 2013 Sep 20;250:43–51. doi: 10.1016/j.expneurol.2013.09.009

Figure 5.

Figure 5

Fibrinogen leakage in the acute and chronic stages of EAE is largely restricted to venules. A. Dual IF for CD31 (Alexa Fluor-488, green) and α-SMA (Cy3, red) was performed on frozen sections of cervical spinal cord. Scale bar =100 μm. Note that α-SMA strongly labels arterioles (labeled A) but is barely detectable in venules (labelled V). B. Dual IF for fibrinogen (Alexa Fluor-488, green) and α-SMA (Cy3, red) was performed on frozen sections of cervical spinal cord taken from 21 (acute) and 35 day (chronic) EAE tissue. Scale bar =100 μm. Note that in the acute and chronic stages of EAE, diffuse extravascular fibrinogen leakage was predominantly associated with venules (arrowheads), rather than arterioles (arrows). C. Quantification of fibrinogen leakage from arterioles and venules at different stages of EAE development. Data points represent the mean ± SEM of 3 experiments. Note that fibrinogen leakage occurs predominantly from venules, particularly in chronic EAE (35 day time-point). * P<0.05, ** p < 0.01.