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. 2013 Jul 30;2(3):520–532. doi: 10.3390/pathogens2030520

Figure 5.

Figure 5

Electrophoretic analysis and differential sensitivity to proteolysis of PrPres in various experimental prion diseases of transgenic mice overexpressing bovine PrP. Transgenic mice Tg110 overexpressing bovine PrP were inoculated with brain tissue from cattle, primate, or Tg650 mice experimentally infected with cattle-adapted TME, L-BSE or c-BSE, or brain tissue from a vCJD patient. The brains were homogenized and PrPres was purified with high concentrations of proteinase K, and detected with monoclonal antibodies that recognize either the octapeptide region (Saf-32) or the core (Bar-233) of the protein. The membrane blotted with Saf-32 was overexposed compared to the membrane blotted with Bar-233.