Distributions of weighted UniFrac distance of skin microbiome, by measures of hand hygiene, participant age and time of sample collection. Surgical intensive care unit healthcare workers participating in the Healthy Hands Study, July, 2011 (N = 34 healthcare workers; N = 102 samples). Panel A: Frequency of alcohol rub use (F statistic for means: 1.8, p-value = 0.13; F statistic for variances: 8.17, p-value < 0.0001); Panel B: Frequency of handwashes (F statistic for means: 4.25, p-value: 0.0053; F statistic for variances: 7.27, p-value < 0.0001); Panel C: Number of gloves donned (F statistic for means: 7.04, p-value = 0.0001; F statistic for variances: 10.05, p-value < 0.0001); Panel D: Age (F statistic for means: 1.22, p-value = 0.30; F statistic for variances: 2.75, p-value = 0.06); and, Panel E: Time within shift (F statistic for means: 2.79, p-value = 0.06; F statistic for variances: 1.31, p-value = 0.27).