Table 2.
Primer sets used in the real-time qPCR assays to detect Enterococcus spp., Staphylococcus aureus, methycillin resistant Staphylococcus aureus (MRSA), and Candida albicans.
| Target | Primers (5' → 3') | Reference | |
|---|---|---|---|
| Staphylococcus aureus | Nuc | GCGATTGATGGTGATACGGTT AGCCAAGCCTTGACGAACTAAAGC |
[24] |
| MRSA | mecA|orfX | TATGATATGCTTCTCC AACGTTTAGGCCCATACACCA |
[25] |
| Enterococcus spp. | 16S | CCCTTATTGTTAGTTGCCATCATT ACTCGTTGTACTTCCCATTGT |
[26] |
| Candida albicans | 18S | GGATCGCTTTGACAATGG GCGGGTAGTCCTACCTGATTT |
[27] |
* Enterococcus faecalis, E. faecium, E. asini, E. saccharolyticus, E. casseliflavus, E. gallinarum, E. dispar, E. flavescens, E. hirae, E. durans, E. pseudoavium, E. raffinosus, E. avium, E. malodoratus, E. mundtii, E. azikeevi, E. canis, E. gilvus, E. haemoperoxidus, E. hermanniensis, E. moraviensis, E. pallens, E. phoeniculicola, E. villorum, E. rottae.