Figure 1.

UT2 Regulates the mTORC2/AKT/FOXO Axis in Primary Hematopoietic Cells

(A) Primary hematopoietic cells from cells overexpressing or depleted for UT2 were analyzed by western blotting using the indicated antibodies. ACTIN was used as a loading control (n = 2–3 experiments).

(B) Graphs show the fold change of the indicated normalized protein ratios (pAKT^S473/AKT and RICTOR/ACTIN) in (A). Data are means ± SEM (n = 3 experiments).

(C and D) Flow cytometry was performed on hematopoietic cells overexpressing or shRNA-depleted for UT2. Quantification of the fold change in mean fluorescence intensity for the indicated phosphoprotein in these cells is shown. Data are means ± SEM (n = 2–3 experiments; two-tailed, unpaired t test). ^∗p < 0.05, ^∗∗p < 0.01.

See also Figure S1.