Table 2.

Differentially regulated phosphopeptides involved in AKT/insulin signaling (1), transcription/translation (2), actin cytoskeleton signaling (3), caveolar and vesicle transport (4) and regulation of apoptosis (5).

Function	ID	Protein Name	Phosphorylation Site in Protein	Baseline Control vs. Insulin Stimulated Control	Baseline Control vs. PMI 5011 Treated	PMI 5011 Treated vs. PMI 5011 Treated and Insulin Stimulated	Insulin Stimulated Control vs. PMI 5011 Treated and Insulin Stimulated	Baseline Control vs. PMI 5011 Treated and Insulin Stimulated
1	P04792	Heat shock protein beta-1 (HSPB1)	S(82)	1.00	1.24	−1.02	1.22	1.22
1	P07900	Heat shock protein HSP 90-alpha (HSP90AA1)	S(263)	1.05	1.27	−1.10	1.11	1.16
1	P08670	Vimentin	S(39)	1.01	−1.03	1.30	1.24	1.26
1	P50502	Hsc70-interacting protein	S(75), S(76), S(79)	1.23	1.42	−1.41	−1.22	1.01
1	Q02952	A-kinase anchor protein 12 (AKAP12)	S(645), S(648), S(651)	1.25	1.33	−1.22	−1.15	1.09
1	Q15121	Astrocytic phosphoprotein PEA-15	S(116)	−1.03	1.23	−1.09	1.16	1.12
1	P02765	Alpha-2-HS-glycoprotein	S(138)	1.45	1.00	1.30	−1.11	1.30
2	O95218	Zinc finger Ran-binding domain protein 2	S(153)	1.22	1.52	−1.28	−1.03	1.18
2	P05387	60S acidic ribosomal protein P2	S(101), S(104)	1.03	1.37	−1.13	1.18	1.21
2	P05388	60S acidic ribosomal protein P0	S(304), D(317)	1.11	1.03	1.23	1.15	1.27
2	P07910	Heterogeneous nuclear ribonucleoproteins C1/C2	S(260)	−1.28	−1.58	1.29	1.04	−1.23
2	P17096	High mobility group protein HMG-I/HMG-Y	S(102), S(103)	−1.33	−1.10	−1.05	1.15	−1.16
2	P19338	Nucleolin	S(145), S(153)	−1.11	1.37	1.02	1.55	1.40
2	P29692	Elongation factor 1-delta (EEF1D)	S(162)	1.02	1.22	−1.19	1.01	1.03
2	P29692	Elongation factor 1-delta (EEF1D)	T(147), S(162)	−1.02	1.21	−1.33	−1.07	−1.09
2	Q13263	Transcription intermediary factor 1-beta	S(19)	−1.18	−1.95	−1.27	−2.10	−2.48
2	Q13283	Ras GTPase-activating protein-binding protein 1	S(232)	1.11	1.19	−1.33	−1.24	−1.12
2	Q15185	Prostaglandin E synthase 3	S(113)	1.33	1.42	−1.39	−1.30	1.02
2	Q15637	Splicing factor 1	S(80), S(82)	−1.14	−1.65	1.60	1.11	−1.03
2	P20042	Eukaryotic translation initiation factor 2 subunit 2 (EIF2S2)	D(4)	1.44	1.82	−1.58	−1.24	1.16
2	O76021	Ribosomal L1 domain-containing protein 1	S(361)	2.99	1.31	−1.32	−3.02	−1.01
3	P46821	Microtubule-associated protein 1B (MAP1B)	S(831), S(832)	1.01	1.24	−1.04	1.18	1.19
3	P50479	PDZ and LIM domain protein 4	S(112)	1.11	1.27	−1.31	−1.15	−1.04
3	Q66K74	Microtubule-associated protein 1S (MAP1S)	T(638), S(640)	1.08	1.34	−1.53	−1.24	−1.15
3	Q9UHB6	LIM domain and actin-binding protein 1 (LIMA1)	S(686)	1.19	1.28	−1.04	1.04	1.23
4	O43493	Trans-Golgi network integral membrane protein 2	S(71)	1.13	1.38	−1.35	−1.11	1.02
4	O60763	General vesicular transport factor p115	S(942)	−1.06	1.45	−1.37	1.12	1.06
4	P08648	Integrin alpha-5	S(127)	−1.24	−1.21	1.27	1.29	1.04
4	P13861	cAMP-dep. protein kinase II-a regulatory subunit	S(78), S(80)	1.17	−1.06	1.02	−1.22	−1.04
4	P27824	Calnexin	S(583)	1.04	1.25	−1.23	−1.02	1.02
4	P35606	Coatomer subunit beta (COBP2)	S(859)	1.08	−1.56	1.20	−1.40	−1.30
4	P51636	Caveolin-2	S(20), S(23)	1.02	1.39	−1.54	−1.13	−1.11
4	Q14247	Src substrate cortactin	T(401), S(405)	2.13	−1.21	1.16	−2.21	−1.04
4	Q14315	Filamin-C	S(2233)	2.19	1.26	2.01	1.15	2.53
4	Q6NZI2	Polymerase I and transcript release factor	S(300)	−1.31	1.08	−1.42	−1.00	−1.32
4	Q969G5	Protein kinase C delta-binding protein (Cavin 3)	S(165), S(166)	1.36	1.71	−1.51	−1.21	1.13
4	Q969G5	Protein kinase C delta-binding protein (Cavin 3)	S(151), S(166)	1.42	2.57	−1.76	−1.01	1.56
4	Q969G5	Protein kinase C delta-binding protein (Cavin 3)	S(151), S(165)	1.15	1.57	−1.76	−1.29	−1.12
5	P25788	Proteasome subunit alpha type-3	S(250)	−1.11	1.26	−1.33	1.05	−1.05
5	P46937	Yorkie homolog (YAP)	S(105), S(109)	1.23	1.78	−1.82	−1.26	−1.02
5	Q9H3N1	Thioredoxin-related transmembrane protein 1	S(247)	−1.14	1.22	−1.37	1.02	−1.12

Shaded values signify statistically significant ratios (p-value < .05).