Table 4.
reagents | media | concentration | temperature | time |
---|---|---|---|---|
1,6-diaminohexane | PBS and DMF / ethanol (10:90 v/v) | 10 mM | 37 °C and 60 °C | 12 h and 5 h |
2-(2-aminoethoxy) ethanol | ethanol | 100 mM | 37 °C | 12 h |
peptides | PBS | 0.7 - 1 mM | 37 °C | 6 h |
reagents | media | concentration | temperature | time |
---|---|---|---|---|
1,6-diaminohexane | PBS and DMF / ethanol (10:90 v/v) | 10 mM | 37 °C and 60 °C | 12 h and 5 h |
2-(2-aminoethoxy) ethanol | ethanol | 100 mM | 37 °C | 12 h |
peptides | PBS | 0.7 - 1 mM | 37 °C | 6 h |