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Purification of crude extract for cellulase isolated from Bacillus VITRKHB
| Purification steps | Total protein (mg/ml) | Enzyme activity (U/ml) | Specific activity (U/mg) | Fold purification | Yield (%) |
|---|---|---|---|---|---|
| Crude extract | 3.10 | 3.80 | 1.225 | 1 | 100 |
| Ammonium sulfate precipitation | 2.14 | 3.43 | 1.60 | 1.30 | 90.2 |
| Dialysis | 1.60 | 3.05 | 1.90 | 1.55 | 80.2 |
| Ion exchange | 0.80 | 1.57 | 1.962 | 1.60 | 41.3 |
