Fig. 2.

Effect of A. bidentata extract (AE) on the cell viability and mineralization of osteoblasts in the presence of antimycin A (AMA). The cells were treated, at confluence, with culture medium containing 5 mM β-glycerophosphate and 50 μg/ml ascorbic acid to initiate differentiation. After 6 days or 14 days for MTT assay (a) and mineralization (b), respectively, the cells were pre-incubated with AE for 1 h before treatment with 70 μM antimycin A for 48 h. Data were expressed as percentage of control. *P < 0.05; control versus AMA, ^# P < 0.05; AMA versus AE