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Co-cultured MDSCs and smooth muscle cells. (a) ×100; (b) ×200: DAPI-labeled MDSCs with blue-stained nuclei (green arrows) and smooth muscle cells with unstained nuclei (red arrows). c Immunofluorescence staining showing expression of α-SMA in the DAPI-labeled MDSCs (green arrows) and co-cultured smooth muscle cells (red arrows). d No α-SMA expression was detected in the DAPI-labeled MDSCs in the control group. (Color figure online)
