Fig. 9.
Expression levels of the transcription factors Oct4, Nanog, Sox2, and Klf4 during human embryonic stem cell differentiation. Three types of human embryonic stem cells and their derived cells (the undifferentiated human embryonic stem cell line hES-T3 (T3ES), hES-T3 derived embryoid bodies (T3EB) and hES-T3 differentiated fibroblast-like cells (T3DF)) were selected for RNA extraction. Expression data were treated with the log2 method. RNA extraction was used from cultured and differentiated cells: the methods have been described in the materials and methods section