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. 2014 Nov 18;9(11):e106143. doi: 10.1371/journal.pone.0106143

Figure 4. Promoter hypermethylation of SFRP1 gene in BS-PCR HRM. SFRP1 is hypermethylated in α-SMA positive myofibroblasts in CRC.

Figure 4

A: Melting peaks of SFRP1 BS-PCR products. Melting temperature (Tm) of 0% methylated standard sample was 77.1°C, while Tm of 100% methylated standard was 79.6°C. B: There was only one unmethylated melting peak detectable for NAT samples (green curves). In CRC samples (red curves) an additional melting peak belonging to hypermethylated products appeared, suggesting that α-SMA positive myofibroblasts are heterogenous concerning SFRP1 promoter methylation.