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. 2014 Nov 18;9(11):e111687. doi: 10.1371/journal.pone.0111687

Figure 3. Validation of Sorcin, Cofilin-1, Apolipoprotein-A1 (Apo-A1) and Ran GTP-binding nuclear protein (Ran) in infertile women.

Figure 3

Expression of Sorcin, Cofilin-1, Apo-A1 and Ran were analyzed by western blot analysis in early-secretory (LH+2) and mid-secretory phase (LH+7) endometrium of infertile women, which had not been previously analyzed in the 2D-PAGE analysis. The results matched the findings of 2D-PAGE analysis. Representative images (upper panel) of immunoblot of Sorcin, Cofilin-1, Apo-A1 and Ran have been shown. β-actin served as a loading control for normalization. Quantification of band intensity (lower panel) was performed by densitometric analysis by using Quantity One software (v. 4.5.1) and a Gel Doc imaging system (Bio-Rad). P values are (b) p<0.01 and (c) p<0.05 versus early-secretory phase (LH+2). Values are expressed as mean± SE; n = 4. ES = early-secretory phase, MS = mid-secretory phase, LH+2 = 2 days after luteinizing hormone surge, LH+7 = 7 days after luteinizing hormone surge.