Table 5.
Titration by qPCR AAV2RSM Versus AAV8RSM
| Laboratory | Replicate | Genome titer AAV2RSM (qPCR) (vg/ml) | Genome titer AAV8RSM (qPCR) (vg/ml) |
|---|---|---|---|
| A | 1 | 2.37×1010 | 7.91×1011 |
| 2 | 1.37×1010 | 4.86×1011 | |
| 3 | 2.83×1010 | 5.39×1011 | |
| Mean | 2.19×1010 | 6.05×1011 | |
| B | 1 | 3.14×1010 | 5.28×1011 |
| 2 | 2.03×1010 | 2.26×1011 | |
| Mean | 2.58×1010 | 3.77×1011 | |
| C | 1 | 1.56×1011 | 4.55×1012 |
| 2 | 2.60×1011 | 6.20×1012 | |
| C′ | 1′ | 3.18×1010 | 5.76×1011 |
| 2′ | 3.11×1010 | 5.58×1011 | |
| 3′ | 3.40×1010 | 5.60×1011 | |
| Mean | 3.23×1010 | 5.64×1011 | |
| D | 1 | 2.00×1010 | 4.10×1011 |
| 2 | 3.80×1010 | 4.20×1011 | |
| 3 | 4.00×1010 | 9.10×1011 | |
| mean | 3.26×1010 | 5.80×1011 |
qPCR, quantitative polymerase chain reaction; RSM, Reference Standard Materials.
According to Lock et al. (2010), AAV2RSM qPCR titer with 95% confidence limits is 2.70×1010 vg/ml to 4.75×1010 vg/ml.
According to Table 4 in this article, AAV8RSM qPCR titer with 95% confidence is 3.05×1011 vg/ml to 1.09×1012 vg/ml.