Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jul 3;23(23):2862–2874. doi: 10.1089/scd.2013.0470

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright 2014, Mary Ann Liebert, Inc.

PMC Copyright notice

FIG. 5. — Epigenetic analysis of the putative promoter of Lrrc34. (A) Methylation analysis of the predicted promoter region of Lrrc34 in differentiated and undifferentiated maGSCs and ESCs by bisulfate pyrosequencing showed the expected hypomethylation of about 8%–18% in undifferentiated and a hypermethylation of about 38%–43% in differentiated maGSCs and ESCs. Depicted is the mean methylation of two biological with each two to three technical replicates. (B) Analysis of histone modification levels at the predicted Lrrc34 promoter in ESCs. The chromatin of ES-RI line was subjected to ChIP analysis using antibodies against the four indicated different histone modifications. The precipitated DNA was analyzed by qRT-PCR for abundance of the various modifications at the predicted Lrrc34 promoter region. The qRT-PCR data are presented as percentage of input DNA. Data are depicted as the mean of two biological with each two technical replicates.