Figure 1. Generation of Pdia3 knockout mouse.

A schematic representation of ablation of Pdia3 gene using gene trap vector inserted into the intron 1 of mouse Pdia3 gene. The exons are numbered and indicated by solid blue boxes. Primers used for genotyping are indicated as a, b and c (A). PKC activity of 1,25(OH)₂D₃ treated wild type and RST613 cells (B). *P<0.05 vs. vehicle treatment, ^# P<0.05 vs. wild type. The samples collected from E10.5 embryos were verified using genotyping (C), RT-PCR (D), western blot (E), and X-gal staining (F).