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. 2014 Nov 18;9(11):e113175. doi: 10.1371/journal.pone.0113175

Figure 1. Effects of pretreatment with estradiol and progesterone on the mammary gland development in MMOC.

Figure 1

WT, αERKO and βERKO mice were treated with 1 µg estradiol and 1 mg Progesterone subcutaneously for 9 days. The thoracic pairs of glands were dissected and cultured in serum free Weymouth’s medium for 10 days in the presence of insulin (1 µg/ml), prolactin (5 µg/ml), aldosterone (1 µg/ml) and hydrocortisone (1 µg/ml). Results show that αERKO mice pretreated with E and P do not respond to hormones, no ductal expansion or alveolar development is observed (panel B), whereas WT and βERKO mice respond to E plus P treatment and to hormones in MMOC.