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. 2014 Nov 18;9(11):e113702. doi: 10.1371/journal.pone.0113702

Figure 1. PCR results for EBV subtyping from the EBNA-3C gene (upper) and for the 30 bp deletion LMP-1 variant (middle), and sequence analysis results for wild-type LMP-1 and the 30 bp deletion LMP-1 variant (lower Upper panel: the primers generated a specific 153 bp fragment from specimens containing type A EBV (samples 1, 2, 4, 5, and positive control PC-2) and a specific 246 bp fragment for specimens containing type B EBV (samples 3, 6, 7, and PC-1).

Figure 1

Middle panel: a specific 316 bp band showed wild-type LMP-1 (samples 3, 4, 6, 7, and positive control PC-1) and a specific 286 bp band indicated the 30 bp deletion LMP-1 (samples 1, 2, 5, and PC-2). The products of positive controls were confirmed by direct sequencing. Lower panel: the 30 bp deletion sequence of del-LMP-1 from wild-type LMP-1 by sequence analysis.