Figure 4. AZGPI quantified mRNA expression for A) HCF and B) HKC cell types.

Four conditions were tested: Control, TGF-β1, TGF-β2, and TGF-β3. In HCFs all three TGFβ isoforms resulted in significant down regulation of AZGPI expression. TGFβ1 showed the lowest of the three when compared to the controls. In HKCs, no significance changes in AZGPI expression was shown for TGFβ1 and TGFβ2 isoforms. TGFβ3 stimulation significantly down regulated AZGPI protein expression. All error bars represent standard error (SEM) of n = 3.