Figure 2. The effect of H2S on Nrf-2-mediated signaling pathway.
Relative mRNA levels of Nrf-2 were assayed in the young and old rats. Pretreatment with NaHS (20 µmol/kg) significantly increased Nrf-2 mRNA (A) and protein (B) levels in the young rats treated with I/R, but it had little effect on those in the old rats. Pretreatment with 20 µmol/kg NaHS significantly increased mRNA (C) and protein (D) levels of NQO1, GST, and HO-1 in the young rats treated with I/R. Pretreatment of 20 µmol/kg NaHS slightly increased mRNA (E) and protein (F) levels of NQO1, GST and HO-1 in the old rats treated with I/R. **P<0.01, indicate significant differences from the respective control groups.