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. 2014 Nov 18;9(11):e113345. doi: 10.1371/journal.pone.0113345

Figure 2. Effects of ORN position on inhibition of PCR amplification.

Figure 2

(A) Positions of ORNs in the target region. (B–D) Dose responses of ORNs. Real-time PCR amplification in the presence of various concentrations of ORNs was normalized against the value in the absence of ORNs. Dose-response curves were generated using the Prism software; Prism was not able to generate a curve of the reference for ORN-666R (B). Data are represented as means ± S.D. (n = 3). P-values for the differences in amplification between the reference and the target at an ORN concentration of 10 nM were calculated using Student’s t-test (B–D).