Figure 3. Inhibitory effects of multiple ORNs on PCR amplification.

(A) Positions of ORNs in the target region. (B) Dose responses of multiple ORNs (ORN-302F and -363R). Real-time PCR amplification in different concentrations of ORNs was normalized against the value in the absence of ORNs. Dose-response curves were generated using the Prism software. Data are represented as means ± S.D. (n = 3). P-values for the differences in amplification between the reference and the target at a total ORN concentration of 20 nM (i.e., 10 nM for each ORN) were calculated using Student’s t-test.