Figure 4. ORN-mediated inhibition of PCR amplification in a competitive setting.

(A) Scheme for ORNi-PCR in a competitive setting. ORNi-PCR can be used for specific inhibition of target sequences while allowing amplification of reference sequences containing the same primer sequences. We designed an ORN targeting the multiple cloning site (MCS) of pBluescript-SK+ (pBS) (ORN-MCS). PCR amplification of the target and reference sequence in the presence or absence of different concentrations of ORNs, using KOD polymerase (B), Pfu polymerase (C), or Taq polymerase (D). Gel images (upper panels) are representative of three independent experiments. The lower panels show dose-response curves of ORN-mediated inhibition.