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. 2014 Nov 19;34(12):2554–2562. doi: 10.1161/ATVBAHA.114.304717

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© 2014 The Authors. published on behalf of the American Heart Association, Inc., by Wolters Kluwer.

© 2014 The Authors.

Arteriosclerosis, Thrombosis, and Vascular Biology is published on behalf of the American Heart Association, Inc., by Wolters Kluwer. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDervis License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited and is not used for commercial purposes.

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Figure 3. — The antiapoptotic effect of CX3C chemokine fractalkine (CX3CL1) is independent of CX3CR1 genotype. Genomic DNA was isolated from a subgroup of donors used in Figure 1C and genotyped for the 2 nonsynonymous single-nucleotide polymorphisms (SNPs) V249I and T280M in CX3CR1 using a polymerase chain reaction–based allelic discrimination assay. Only the 3 most common haplotypes were identified, and the response to CX3CL1 was analyzed by genotype. For each donor in each experiment, the response to serum-starvation was normalized to 100% and the effect of CX3CL1 expressed as a fraction of this to minimize technical variation between experiments. Data shown as mean±SEM, n=34 donors. No significant difference between genotypes was detected using 1-way ANOVA. AAD indicates 7-aminoactinomycin D.