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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Am J Transplant. 2014 Oct 30;14(12):2691–2703. doi: 10.1111/ajt.12934

Figure 5. Loss of phenotypic integrity of infused Tregs after adoptive transfer.

Figure 5

Figure 5

Persisting infused cells in the peripheral circulation were identified by CD3, CD4 and CFSE positivity and were then analyzed for the expression of CD25, FoxP3 and CD127.

A. Representative flow cytometric analysis of FoxP3 and CD25 expression in CD3+CD4+ CFSE+ cells over time.

B. Combined longitudinal analysis of CD25+FoxP3+ cells in the total CD3+CD4+CFSE+ population (mean ± SEM of three independent experiments).

C. Combined longitudinal analysis of CD25+ cells in the total CD3+CD4+CFSE+ population (mean ± SEM of three independent experiments).

D. Combined longitudinal analysis of FoxP3+ cells in the total CD3+CD4+CFSE+ population (mean ± SEM of three independent experiments).

E. Combined longitudinal analysis of CD127−/low cells in the total CD3+CD4+CFSE+ population (mean ± SEM of three independent experiments).