Figure 3. Mitochondrial biogenesis in failing hearts.
Total ventricular protein from NF, ICM and DCM ventricles assayed by (A) immunoblot and (B) quantified for mt biogenesis regulators PGC-1α and c-Myc. (*P<0.05 vs NF; (n=4/group). (C) Real-time PCR from NF and failing ventricles for mt biogenesis genes POLG, POLG2 and TFAM. (*P<0.05, **P<0.05 vs NF; n=8/group). (D) Total ventricular lysates from NF, ICM and DCM probed with antibodies against NRF-1, POLG and POLG2 along with GAPDH as a loading control (n=4/group).